The Single Best Strategy To Use For flow cytometer

The Single Best Strategy To Use For flow cytometer

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Data-enriched characterization of cell populations. Morphological info from photographs can add on the richness of flow cytometry details. By way of example, the figure demonstrates an otherwise standard apoptosis assay applying Annexin V and PI, incorporating cell imaging to characterize cells in Each and every inhabitants to reveal morphologically unique features. These insights couldn't have been attained from multiplex staining alone.

Irrespective of whether you’re a starter or an expert, take a look at our assets to progress your study and experience in flow cytometry.

Merge acoustic focusing technological know-how with higher-parameter Assessment for adaptable spectral unmixing and conventional workflows.

The award-profitable Invitrogen Attune CytPix Flow Cytometer combines acoustic focusing with a large-speed brightfield digital camera for simultaneous large throughput flow cytometry and significant resolution brightfield imaging.

*Pricing is indicative only which is subject matter to change. Be sure to Speak to your Agilent representative with any queries. The information established forth herein is not really a suggestion or perhaps a dedication, equally of that are subject to credit approval, conditions and terms, and availability with Agilent. Agilent Economic Methods is out there in chosen countries.

Here, a highly trained consumer has gated singlets confidently. After analyzing the handbook singlet gate, the CytPix impression-derived parameter ParticleCount reveals this gate consists of a lot more than four% aggregates.

Pair your Attune flow cytometer using an autosampler for amplified workflow efficiency, and increase robotic integration for max throughput.

Dependable graphic quality even at substantial throughput Acoustic concentrating and also a higher-pace digital camera Incorporate to image these T cells continually at minimal or high flow charges. Simply regulate concentrate and digicam options to meet experimental specifications.

Early signs of this contamination experienced initially been dismissed as particles. Backgating to find new populations. Backgating imaged cells to the Attune CytPix Flow Cytometer also lets you use morphological functions to find out attention-grabbing subpopulations that would not be obvious from flow cytometry details by yourself.

Perhaps most importantly, these functions have cells of clearly diverse phenotypes which could lead to incorrect conclusions about double good functions (specifically in uncommon populations).

Audio waves enable precise mobile alignment, resulting in Fantastic data good quality and ~10X more quickly sample flow costs.

Software Take note: Automatic impression analysis lessens user-to-consumer variability in flow cytometry gating methods

Constructed-in dimensionality reduction instruments increased resolution into populations. Fluorescently gated populations back-gated onto UMAP plot to confirm predicted populace resolution in lysed whole human blood. Consumer can look at functions from Each and every cluster for visual confirmation of mobile phenotypes.

Flexible payment prepare from Agilent Money Options enable you to obtain the essential instrumentation you may need for rapid job, or to increase your lab.

At the heart of every Attune Flow Cytometer lies our revolutionary acoustic concentrating technological know-how, which makes use of audio waves to meticulously align cells in the flow cell. This progressive solution surpasses standard hydrodynamic approaches, causing quicker processing speeds, Extraordinary information high-quality, and greater sample throughput.

As the cell line had Earlier been analyzed to the Attune CytPix Flow Cytometer, the scientists went back to the photographs and had been in the position to document the microbial infection no less than 5 days earlier.

Agilent Benefit Guarantee guarantees you not less than a decade of instrument use in the date you purchase. Usually, We are going to credit you with the residual worth of the technique towards an upgraded product.

Below, we display that by gating tactics here using graphic-based mostly quantitative parameters can distinguish interacting cells from coincident gatherings to additional correctly evaluate interacting cells.